Journal: Regenerative Biomaterials

Article Title: Engineered chitosan for improved 3D tissue growth through Paxillin-FAK-ERK activation

doi: 10.1093/rb/rbz034

Figure Lengend Snippet: Comparison between AM functionalization strategies on chitosan scaffold for enhancing cell adhesion and proliferation

Article Snippet: Human mesenchymal stem cell (hMSC) (UE6E7T-3, Riken Bioresource Center) was cultured and maintained in standard growth medium (MEMα) supplemented with FBS (10%) and Pen-Strep (1%).

Techniques: Comparison

Journal: Regenerative Biomaterials

Article Title: Engineered chitosan for improved 3D tissue growth through Paxillin-FAK-ERK activation

doi: 10.1093/rb/rbz034

Figure Lengend Snippet: ( a ) Schematic elastration of RGD functionalization of chitosan. SEM images of prestine chitosan scaffold ( b ) and RGD functionalized chitosan surface ( c ). Scale bar for main images 30 μm and scale bar for inserted images 3 μm. ( d ) Evaluation of adhesion strength of hMSC cells on RGD functionalized chitosan scaffold and pristine chitosan scaffold after 6, 12, 18 and 24 h post seeding. ( e ) Proliferation of hMSC cells on RGD functionalized chitosan scaffold as well as pristine chitosan scaffold at 1, 7, 14, 21 and 28-day post seeding. All values are expressed as mean ± standard deviation from five individual samples. Asterisk indicates significant difference (** P < 0.01and * P < 0.05) as compared with RGD non-functionalized samples

Article Snippet: Human mesenchymal stem cell (hMSC) (UE6E7T-3, Riken Bioresource Center) was cultured and maintained in standard growth medium (MEMα) supplemented with FBS (10%) and Pen-Strep (1%).

Techniques: Standard Deviation

Journal: Regenerative Biomaterials

Article Title: Engineered chitosan for improved 3D tissue growth through Paxillin-FAK-ERK activation

doi: 10.1093/rb/rbz034

Figure Lengend Snippet: ( A ) SEM characterization of cell-cultured scaffold: images obtained from cell-cultured pristine chitosan scaffold fabricated (a, c, e and g) and RGD functionalized chitosan scaffold (b, d, f and h) of which a and b obtained at day 7, c and d at day 14, e and f at day 21 and g and h at day 28 of post seeding. Scale bar 30 μm. ( B ) Histochemistry of hMSC-cultured scaffold: H&E stained images of histologic section obtained from cell-cultured pristine chitosan scaffold (a, c, e and g) and RGD functionalized chitosan scaffold (b, d, f and h) of which a and b obtained at day 7, c and d at day 14, e and f at day 21 and g and h at day 28 of post seeding. Images were obtained using 10× objectives

Article Snippet: Human mesenchymal stem cell (hMSC) (UE6E7T-3, Riken Bioresource Center) was cultured and maintained in standard growth medium (MEMα) supplemented with FBS (10%) and Pen-Strep (1%).

Techniques: Cell Culture, Staining

Journal: Regenerative Biomaterials

Article Title: Engineered chitosan for improved 3D tissue growth through Paxillin-FAK-ERK activation

doi: 10.1093/rb/rbz034

Figure Lengend Snippet: Effect of hMSC proliferation on compressive behavior of porous chitosan scaffold. All values are expressed as mean ± standard deviation from three individual samples. The significant difference ( P < 0.05) was compared with pristine scaffold without hMSC

Article Snippet: Human mesenchymal stem cell (hMSC) (UE6E7T-3, Riken Bioresource Center) was cultured and maintained in standard growth medium (MEMα) supplemented with FBS (10%) and Pen-Strep (1%).

Techniques: Standard Deviation